Regardless of your physical RAM, only a limited amount of RAM is available during the early stages of boothing up; apparently only about 4MB. Occasional conflicts with some PCI cards have been reported, particularly on older systems. Unfortunately this problem does not appear to have been addressed by Apple, and occasionally an upgrade to a newer OS will suddenly cause your machine to refuse to boot up. If you experience a problem booting with a new OS, and have several PCI cards plugged in to slots on your machine, you may have to learn to live without one to use your new system!

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